For detailed protocols related to human gastrointestinal biopsy grown enteroids, go to www.umichttml.org/protocols

SPENCE LAB PROTOCOLS 

(Click for detailed documents)

Shipping and receiving live spheroids

Cell lines for producing growth factor conditioned media

Generating Growth Factor Conditioned Media from Wnt3a, RSpo1, Rspo2 and Nog cell lines

Lentiviral transduction of Enteroids

single cell dissociation of human tissues for FACS

Human Pluripotent Stem Cell Electroporation Protocol

Single cell dissociation of human tissue for single cell RNA sequencing

A Method for Cryogenic Preservation of Human Biopsy Specimens and Subsequent Organoid Culture

General Whole Mount Immunofluorescent Staining Protocol

OLFM4 Staining Protocol

4% PFA Protocol

Making Citrate and Sodium Citrate Buffers